Electron Microscopy Analysis

The Center of Bioinformatic and Molecular Simulation (CBSM) from Talca University has a LVEM5 microscope.  I’m in charge of this unit and we perform SEM and TEM analysis for a broad type of samples with the MS. Yerko Argandoña.

The LVEM5 is the first Low Voltage Electron Microscope, built on a revolutionary technology platform that combines advanced multi-mode imaging with unparalleled benchtop convenience.

The LVEM5 has an architecture that departs from traditional models. The benchtop design alone is a significant architecture & footprint departure from classical TEM design. The LVEM5 is approximately 90% smaller than classical electron microscopes. In contrast to classical architecture, the electron optic column is very short, making up only ~ 50% of the LVEM5 tiny height. This means that the LVEM5 can be installed in a lab, on a desktop or benchtop; almost anywhere electron imaging is needed.

Low-Voltage Field Emission Gun: High brightness and high contrast:  The electron source determines in large measure the parameters of the electron microscope. The uniquely-designed Schottky type field emission gun employed by the LVEM5 has very high brightness and spatial coherency.  TEM Image contrast is given by varying degree of interactions between electrons in various parts of the specimen. Low energy electrons interact much more strongly with the sample than high energy electrons of classical TEM. Electrons in the LVEM5 are strongly scattered by both inorganic and organic materials, resulting in exceptional differentiation of features. The density difference required to give 5% contrast difference at 5kV is a mere 0.074 g/cm3

• Bacterium (SEM)

• Bacterium (TEM)

• Blood

• Fungus

• Virus

• Inorganic surface

• Hydrogel

• Nanotube

• Nanospheres